温泉と鉄代謝に関する研究

第2報 1回泉浴による血清鉄の変動動物実験における採血方法についての批判

抄録

Reports are scant concerning the influence of hot-spring bathing upon hematopoietic function.
The author attempted to observe the variation in serum iron by single bathing. Since rabbit serum iron value is changeable by the way of venesection itself and this could be connected with iron metabolism in a case of acute bleeding, the author studies this problem in the preliminary experiment. The results obtained are summarized as follows:
1) In animal experiments (rabbit), it is desirable to limit the number of blood collection to two, and the amount of initial sample to less than 4ml for the observation of serum iron changes to minimize influence of the removal of blood. And it is necessary to give careful consideration to the difference in serum iron values if determination was made after the long interval.
2) In acute bleeding, the decrease of serum iron at the beginning of bleeding was due simply to hydremia, associated with the loss of blood. In the subsequent course, however, serum iron value tended to be considerably complicated by the flow of storage iron into blood stream or the stasis in hematopoietic organs, but, on the whole, serum iron tended to decrease gradually.
Iron binding protein, at the beginning of bleeding, decreased in proportion to serum protein and the rise in the saturation per cent was temporarily resulted from the tendency to maintain constancy in serum iron. Subsequently, the saturation per cent gradually decreased.
And in the early stage such as 2 to 3 days after bleeding, iron binding protein remarkably increased before the recovery of serum protein, and the rate of increase was 25.8per cent.
From the viewpoint of iron absorption, this may be considered to be a vital reaction which serves the purpose.
3) Reports are few concerning the serum iron value of the rabbit, and the author measured the serum iron values in 118 healthy mature rabbits. The results are as follows:
_??_251.4±13.2γ/dl _??_211.5±14.9γ/dl (by Yoshikawa's method),
_??_205.9±14.8γ/dl _??_ 194.1±15.2γ/dl (by Matsubara's method),
_??_198.5±11.4γ/dl _??_199.4±10.9γ/dl (by Noda's method).
4) Single bathing in “Genzc-yu” (acid vitriol spring), “Unagi-yu” (alkaline sulphur spring) and simple carbondioxated spring at Narugo Spring showed no significant influence on the serum iron in healthy persons and rabbits.