⁵⁹Feによる温泉作用の研究

抄録

Section 1: Organ distribution of ⁵⁹Fe following percutaneous absorption of iron by bathing.
The lower half of the body of the mouse was placed in a 500ml beaker containing 150ml of bath water (pH 10) and 200μc of ⁵⁹Fe at 37°C for 30 minutes, The animal was fixed by tying to a wire netting during the bath so as not to drink bath water. Five mice were used in the same experiment. Following the bathing, the animals were washed to remove contaminated ⁵⁹Fe on the skin and then sacrificed by decapitation immediately after, at 3, 6, 12 and 24 hours after bath.
Immediately after decapitation, blood was collected from the neck as much as possible and liver, spleen, kidney, muscle and vertebral bone were removed. The radioactivity of each organ was measured in a scintillation counter with dry combustion method.
The radioactivity was detected in various organs such as blood, liver, spleen, kidney, muscle and bone. These results provide evidence of the percutaneous absorption of radioactive iron into the body.
Section 2: The effect of serial bathing on the concentration of ⁵⁹Fe in organs following percutaneous absorption, gastrointestinal administration and intramuscular injection.
Acid springs from our Balneotherapeutic Institute and distilled water were used for bathing in this experiment.
Test animals were divided into 4 groups, that is, group 1 received bathing for 2 days, group 2 for 4 days, group 3 for 7 days and group 4 for 14 days. The animal was placed in a bamboo tube of which one edge was covered with wire netting. The lower half of the body was soaked in the bath water at 37°C for 10 minutes every day during the experimental period. Animals received no bathing were used as control. On the day following last bathing of each group, the absorption experiment of ⁵⁹Fe were carried out.
It was shown that a variety of result due to individuality was obtained in the first 7 days of serial bathing, and “Habitual Phenomen” or defence mechanism was seen in the groups treated with the serial bathing of 14 days.
Section 3: Autoradiography of the skin bathed in the bath water labeled with ⁵⁹Fe.
One part of the skin of the animal bathed in the bath water labeled with ⁵⁹Fe was excoriated and applied to the autoradiography. In this experiment, Fuji Autoradiographic Plate for stripping method was used.
The autoradiogram showed that the highest concentration of radioactivity was found in the epidermal portion and the density became decreased gradually as approaching to the corium. From this experiment, it was demonstrated histologically that radioactive iron in bath water was absorbed percutaneously into the body.