Physiological effect of sera obtained from an electrically charged human body on giant axons of squid (Doryteuthis bleekeri) was examined by observing action potentials when the axons were extracellularly perfused with a 2:1 mixture solution of artificial sea water (ASW) and sera. Three kinds of sera were prepared; the one was obtained from a negatively-charged human body and the others were from a positively-charged and a non-electrically charged (or normal) human bodies.
Amplitudes of action potentials in axons immersed in ASW containing the sera obtained from a negatively-charged human body first increased with time after the onset of the external perfusion. On the other hand, the amplitudes of action potentials in axons bathed in ASW containing the sera obtained from a positively-charged or a non-electrically charged human body did not increase but became reduced with time after the onset of the perfusion. These suggest that some facters involved in sera obtained from a negatively-charged human body are responsible for improving the function to generate nerve excitation.
One of the facters may be increased number of electron charges involved in the sera from a negatively-charged human body since the flocculation number, which has been known to be inversely proportional to the number of electronic charges in the specimen, was experimentally confirmed to be relatively low in the sera from a negatively-charged human body.
