耳鼻咽喉科展望
Online ISSN : 1883-6429
Print ISSN : 0386-9687
ISSN-L : 0386-9687
EBウイルス関連抗原検出のための新しい螢光抗体染色法
エバンスブルー二重染色法
滝元 徹宮崎 為夫岩脇 淳一石川 滋田中 佐一良桝田 耕作本 真吉崎 智一西村 俊郎
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1987 年 30 巻 1 号 p. 75-78

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The immunofluorescence method (IF) has the advantage of being a relatively simple procedure, and useful for rapid diagnosis of many viralinfections. The Epstein-Barr virus (EBV) serological test by the use of IF is also useful for the diagnosis of nasopharyngeal carcinoma. The indirect IF has been used to detect EBV-specific antigens. Authors devised a combination method between routine IF and Evans Blue staining. The method is often useful for comparison of cell morphology and the site of EBV-specific antigens.
In addition, the use of Pro-Tex ® as a mounting medium can preserve a marked loss of immunofluorescence.
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