抄録
To clarify the mechanism of bone destruction in chronic otitis media with cholesteatoma, bone chips of tympanic bulla of guinea pigs were soaked in the propionic acid solution and it's electric conductance, pH and concentration of Ca2+ & PO43- were measured. The results indicated that neutralization and releasing ions from the bone proceeded simultaneously in the solution. And we could get the equation reflecting well actual bone destruction, suggesting that the rate of bone destruction depends on pH and bone surface area. It was also recognized that these ions such as Ca2+ and PO43- in the solution derived mainly from hydroxyapatite in the bone. Besides, a mass of collagen type II or a bone chip of protein-part was soaked for two weeks in the solution of which conductance indicating bone destruction (demineralization) had reached equilibrium and then samples were observed using laser-Raman spectroscopy. From the results it may be concluded that the remineralization could occur on the surface of the materials like collagen with adsorptive activity in the saturated solution.
