The motion pattern of the transplanted homograft tympanic membrane (HTM) under free-field acoustic stimulation was studied using the time-averaged holography in cats. HTM was obtained, processed and transplanted to the animal just the same as to humans. Present results showed that the amplitude and vibrating area of the transplanted HTM increased in due course of time because the HTM gradually became much thinner after transplantation. The anatomic and histologic observations of transplanted HTM revealed that HTM it was well adapted to the recipient's tympanic annulus and became thinner as time passed by. These results suggest that pars tensa of the transplanted HTM keeps good mobility after homograft tympanoplasty in experimental animals.