Arthus型実験的中耳炎
中耳粘膜の形態学的研究
1989 年 20 巻 1 号 p. 167-168
詳細
1989 年 20 巻 1 号 p. 167-168
An experimental model for otitis media with effusion (OME) induced by type III hypersensitivity reaction using gram-negative bacterial lipopolysaccharide (LPS) in 60 guinea pigs was sudied immunohistochemically.
Serous-type effusion was observed at 2 days after the admini-stration. It changed into the mucoid type on the next day and could be observed until 8 days after administration.
Increase of secretory cells, infilration of many neutro-phils, macrophages and plasmacytes in the tympanic cavity and leucocyte masses in the submucosal layer were observed histo-pathologically.
The localization of LPS, concomitant immunoglobulin such as IgG, and complement C3, were envestigated using peroxidase-labelled antibody method. The localization of immune complex (I. C.) Was determined at the same position on the antigen, immunoglobulin and complement.
The deposition of I. C. Aas observed on the basement membrane of mucous epithelium, endothelium of blood vessels of the submucosal layer and surface of cilia.
