生理活性物質の中耳, 耳管粘膜に対する影響について
第1報: 血小板活性化因子PAFのモルモット耳管粘膜に対する影響
1989 年 20 巻 1 号 p. 367-368
詳細
1989 年 20 巻 1 号 p. 367-368
Until now endotoxin has been identified as possibly playing a role as a pathogenesis of otitis media with effusion. Of paticular interest, is the inhibition of endotoxin induced biological responses by specific PAF antagonists, which has been perviously reported suggesting involvement of PAF in endotoxin induced biological responses. In the present study, PAF induced mucosal damage of the eustachian tubes of guinea pigs was described. The surface profile of the mucosal specimens, which were aseptically obtained under an operating microscope, were observed in Eagle's MEM under a phase-contrast microscope equipped with a thermoregurated humidified CO2 chamber. The profile was monitored on a TV monitermagnified 2, 500x and simulteniously recorded on videotapes. Ciliary activity was photoelectrically measured on the TV moniter. PAF induced ciliary inhibition along with swollen epithelial cells, exfoliation of the cells and eventually ciliostasis at concentrations of 10-6M to 10-10M. In contrast, both of the control groups of methanol and 10-6M lyso-PAF in Eagle's MEM control groups failed to induce any alterations of the mucosal surface profile or changes of ciliary activity.
