Perilymph and endoltmphatic pressures were measured simultaneously using two sets of a servo-nulling system in guinea pigs, and the pressure difference between them associated with subarachnoid infusion was studied. Subarachnoid infusion of saline caused pressure increase in both the perilymph and the endolymph equally in normal guinea pigs. Similar results were obtained in the guinea pigs with surgical obstruction of endolymphatic sac and duct. Reissner's membrane may not have stiffness to hold the pressure difference between them.