Pressure transmission from the external ear to the inner ear was studied in guinea pigs after closure of the Eustachean tube. The pressure was slowly applied to the ear canal to reach a peak of 1000 or -1000 mmH2O. When the positive pressure was applied, pressures of the middle ear and inner ear increased up to around 400 mmH2O. and 200 mmH2O, respectively. When the negative pressure was applied, they decreased to -100 mmH2O and -50 mmH2O, respectively. This means that positive pressure load to the external ear is transmitted more than negative one. The decrease in the inner ear pressure during pressure loading is due to patency of the cochlear aqueduct. Rupture of the round window membrane occurred by the pressure load over 1000 mmH2O.