抄録
Vestibular hair cells were isolated from saccule and ultricle of guinea pig by enzymatic and mechanical dissociation. These hair cells were classified into two types; cells which showed flask shape form and different length of neck are thought to be type I cells and cells which showed rod or round shape form and had no neck might be type II cells. Using these cells, we examined their intracellular distribution of cytoplasmic free Ca2+ concentration ([Ca2+] i) by the Ca2+ sensitive dye fura-2. In the resting state, type I like cell showed high [Ca2+] i in nucleus and cuticular plate although type II like cell showed high [Ca2+] i only in nucleus. During ionomycin stimulation and depolarization state induced by elevated K+, [Ca2+] i of both types of cells were markedly increased.
