モルモット内リンパ嚢の基底膜成分の局在

抄録

The distribution of type IV collagen (C-IV) and laminin, which are important components of basement membrane (BM), in the endolymphatic sac (ES), kidney, spleen and lymph node of healthy Hartley guinea pigs was studied immunohistologically. Antibodies against C-IV and laminin were used in this study. After proteolytic digestion, these components were able to be detected on the paraffin embedded tissue sections. The distribution of C-IV in the examined tissue sections was almost the same as that of laminin whereas C-IV was localized in subepithelial connective tissue of the ES where laminin was not seen. The epithelial BM of the rugose portion of the ES as well as tubular BM of the kidney showed the most intense labeling by anti-C-IV and-laminin antibodies while these components were weakly found in the epithelial BM of the distal portion of the ES. Additionally, C-IV and laminin were confirmed to be localized in vascular BM.
These results suggest that the epithelial BM of the rugose portion may play an important role in fluid transport in the endolymphatic sac.