バゾプレッシンレセプター・ANPレセプター・アルドステロンレセプター遺伝子のラット内耳における発現

抄録

the rat cochlea and endolymphatic sac (ES) was examined by PCR and in situ hybridization.After reverse-transcription of mRNA of the rat cochlea and ES, cDNA was amplified by PCRusing pairs of primers specific to these receptors. After subcloning of the PCR products, the nucleicacid sequences was determined. Next, localization of the expression of these receptors in the cochleaand ES was examined by in situ hybridization. Expression of vasopressin Vla, V2, ANP-A, ANP-B and aldosterone receptors was detected in the cochlea by PCR. Expression of the Vla and ANP-A receptors was detected in the ES by PCR. The expression of following receptors was determinedby in situ hybridization: ANP-A receptor in the cochlear nerve root, ANP-B receptor in thespiral ganglion and aldosterone receptor in marginal cells of the stria vascularis and in epithelialcells of the intermediate portion of the ES. These results suggest that vasopressin, ANP and aldosteroneplay certain roles in the cochlea as well as in the ES. In particular, it seems possible that ANP plays a role in the function of the cochlear nerve and that aldosterone plays a role in the productionand absorption of endolymphatic fluids.