³¹P-NMRを用いた高等植物の細胞内pH測定

抄録

In higher plant cells, measurement of the intracellular pH (cytoplasmic and vacuolar pH) has proved very difficult because of the existence of vacuoles in the cells. Here, we investigated the intracellular pH of higher plant cells (mung bean root tip cells) by using ³¹P-NMR. Estimations of the cytoplasmic and vacuolar pH were made from the P. chemical shifts of cytoplasm and vacuole, respectively. In this paper, we propose a ³¹P-NMR perfusing system. Using this system, we can investigate the cytoplasmic and vacuolar pH and the H⁺ efflux simultaneously at room temperature. As the measurement of intracellular pH using the ³¹P-NMR perfusing system is a noninvasive and nondestructive method, we believe that this method will play an important role in the future study of intracellular pH regulation mechanism.