抄録
Dielectrophoretic spectroscopy using frequency-modulated (FM) electric fields is among simultaneous multi frequency methods in a vast field of spectroscopic researches and is available for one-step electric assay of cells. When the frequency range covers a crossover frequency that is traversed with the reversal of dielectrophoretic direction, the FM field reciprocates cells. The video analysis provides the precise value of crossover frequency within one minute. The Jurkat cells, used as a typical line of leukemia cells, have been found to have a monodisperse and reproducible distribution of crossover frequencies as far as the solvent conductivity is kept constant. Moreover, the FM method has revealed a gradual increase of crossover frequency with aging due to the air-incubation at room temperature, indicating the possibility of electrical assay for high resolution probe of viability.
