Corn cellulose powder (CCP) was produced from corn powder by chemical treatment and bleaching process. CCP was stirred in distilled water over night and exposed to ultrasound (US) 30min with 23kHz, 96kHz, 141kHz, 500kHz consecutively. After the US was exposed at different frequencies, distribution of CCP were measured and particle size of CCP grew smaller along with increasing frequency. TEM images and FT-IR spectra showed that the hydrogen bond between cellulose molecules were destructed by ultrasound. These results indicated that ultrasound is effective in cellulose dispersion in water medium.