歯周疾患における歯肉浸出液中の Bradykininase 活性について

抄録

The subjects in various periodontal conditions were examined based on the clinical symptoms and roentgenograms, and divided into five groups: clinically normal, gingivitis, slight, moderate and advanced periodontitis. The gingival fluid was collected from gingival sulcus by filter paper strips. The amount of gingival fluid and its bradykininase activity were assayed according to Takeya's method. The bradykininase activity was expressed as the amount of gingival fluid (mg) to produce 50% inactivation of added bradykinin (1μg/ml).
The following results were obtained;
1) The quantity of gingival fluid in clinically normal subjects averaged 1.74±0.38mg (mean±S. D.). Subjects with gingivitis, slight periodontitis, moderate periodontitis and advanced periodontitis showed the values of 4.68±3.13mg, 7.56±2.81mg, 10.68±7.67mg, and 19.18±12.89mg, respectively. The quantity of gingival fluid was increased with the progression of periodontal disease.
2) The bradykininase activity in gingival fluid in clinically normal subjects expressed as the amount of gingival fluid to produce 50% inactivation of added bradykinin (1μg/ml) averaged 0.31±0.14mg (mean±S. D.). Subjects with gingivitis, slight periodontitis, moderate periodontitis and advanced periodontitis showed the values of 1.05±0.90mg, 1.47±0.59mg, 2.61±2.59mg and 3.61±2.87mg, respectively. The bradykininase activity was decrease with the progression of periodontal disease.
3) The bradykininase activities in subjects with advanced periodontitis were significantly lower than those of clinically normal (P<0.05) and gingivitis subjects (P<0.05). No statistically significant difference was found between the normal and disease groups.
4) Statistically significant correlations were obtained between the bradykininase activity expressed as the amount of gingival fluid to produce 50% inactivation of added bradykinin (1μg/ml) in diseases, and oral examination; gingival score (P<0.01), plaque score (P<0.05), calculus score (P<0.05), sulcus depth (P<0.001), and quantity of gingival fluid (P<0.001), except bone loss score.
5) THe degrees of gingival score, oral hygiene score, sulcus depth, bone loss score, and quantity of gingival fluid were increased according to the disease process.
It is concluded from these results that the measurement of the kininase activity which is thought to be an important factor for occurence and progression of periodontal disease may be a valuable mean.