ガスクロマトグラフィーによる口腔内揮発性硫化物の分析

抄録

The purposes of this investigation were to research the major component and sources of halitosis. The subjects were based on the clinical findings and the intensity of odour by olfactory panel of trained judges. These were divided into three groups, subjects without halitosis: group N, periodontally healthy subjects with halitosis: group H-N, periodontally diseased patients with halitosis: group H-P.
The test samples were withdrawn with a 5ml gas tight syringe consisting of glass barrel through capillary tubes after closing the mouth and continuing breath through nose for 1min. Entire 5ml mouth air sample was injected directly on to G-C column, and determined by a gas chromatograph equipped with. flame photometric detector to measure the amount of sulphur containing compounds in mouth air.
The results were as follows:
1. The concentrations of volatile sulphur compounds in mouth air were proportional to organoleptic ratings by a panel of trained judges.
2. It seemed that methyl mercaptan was more closely related than hydrogen sulphide to halitosis, and dimethyl sulphide was little related to halitosis judging from the direct determinations of the volatile sulphur compounds, odour threshold and objectionabilities.
3. The tongue coating score of group H-N were remarkably higher than group: N and group: H-P.
4. The pH of whole saliva showed no statistically significance among three experimental groups.
5. In group H-P, methyl mercaptan content in mouth air was observed high correlation with gingival score (P<0.01), but no significant correlation with pocket depth and bone loss.
6. Rinsing, brushing with dentifrice and without dentifrice were temporarily caused a pronounced reduction in sulphur content.
7. It was assumed that main sources of halitosis were periodontal pockets and coated tongue. Surgical elimination of periodontal pockets and mechanical removal of tongue coating were the most effective treatments for halitosis.
It is therefore concluded that halitosis is closely related with the volatile sulphur compounds represented by methyl mercaptan produced in the periodontal pockets and on the coated tongue.