歯肉切除後における新生ヒト歯肉コラーゲンの形態学的及び生化学的分析

抄録

The present study was undertaken to examine biochemically and histologically the relationship between tissue repair and collagen formation after gingivectomy. Two biopsy samples of labial gingival tissue were taken from the same area of each patient with periodontal disease at three times; before, one month and six months after surgery. The neutral salt-soluble and -insoluble collagen content in these biopsy samples was assayed colorimetrically, while a parameter for evaluation of the collagen fibers in 5μ histologic sections stained with Masson-Goldner was expressed in term of numerical density under the multispectrophotometer.
The results were as follows;
1. The amount of soluble collagen extracted 3 times every 24 hours decreased with time.
2. The soluble collagen content before surgery averaged 47.66×10^-2μg/mg (gingival wet weight), and it increased to 68.66×10^-2μg/mg at one month after surgery, and followed by a slight decrease to 60.82×10^-2μg/mg at six months after surgery. This may reflex a more active collagen synthesis in the gingival tissue at one month after surgery.
3. The mean preoperative value of insoluble collagen was 71.83μg/mg and at one month after surgery the amount decreased to 32.83μg/mg, less than half of preoperative value, and then at six months after surgery it returned to 68.49μg/mg, approximately preoperative value.. This may show that the collagen maturation in the gingival tissue repair has completed by six months after surgery.
4. In the present study a close correlation was demonstrated between the insoluble collagen content and the numerical density of collagen fibers in the histologic sections.
5. In the histologic sections before and six months after surgery the collagen fibers were more dense in oral epithelium site than pocket epithelium site, whereas they were found loosely throughout gingival lamina propria of one month postoperative sections. It may be related to the degree of the inflammatory cell infiltration and the amount of mature collagen that the distribution of collagen fibers before and six months after surgery was different from one month after surgery.