The purpose of these experiments are to detect on the stability of dextranase that was absorbed through either oral mucosa or skin of animals.
The methods and materials are described as follows.
Method
One is the radioimmunoassay with 125I (RIA). (limited detection 25ng/ml)
The other is enzyme activity (EA). The absorption through skin were detected with radioimmunoassay, other enzyme activity.
Only the hair of rabbits on the back are trimmed, the skin was injured and dextranase was dressed on it.
The absorption through oral mucosa.
Gingivae in the mandibular anterior portion are applied for this research.
Only control group is covered on lower oral mucosa, test group is injured in the marginal gingivae with dental hand instruments.
The result was obtained as follows.
(1) In control group, dextranase absorption into the tissue was not detected in normal skin, whereas it was detected on the injured skin of rabbits.
(2) Neither normal oral mucosa nor injured one as to dextranase absorption was detected.
(3) Dextranase absorption by means of subcutaneous injection to rabbits was detected decreased rapidly on the half life by two hours.
(4) The absorption of dextranase was not significant factor as to surface active agents.
