抄録
The present study was performed to elucidate the variation of kinin contents and kininase activities in the saliva, plaque and gingival fluid of the healthy people without oral hygiene.
Nine male dental students, aged 20-24, were the experimental subjects. All of the participants had a tooth scaling and polishing, and then they were instructed not to brush their teeth for the following 10 days. At the 11th day, they were allowed to have a tooth brushing (Rolling method) 3 times a days for 5 days. The collection of samples (saliva, plaque and gingival fluid) and clinical examinations were performed daily for the entire experimental period (16 days). Kinin contents and bradykininase activities in the samples were assayed by a biological method of Takeya et al. The bradykininase activity was determined by measuring the percent of inactivation of added bradykinin (1μg/ml).
The following results were obtained;
1. The plaque index, gingival index and amount of gingival fluid were increased throughtout the period without oral hygiene (10 days). These clinical scores showed a rapid decline when the oral hygiene procedure was resumed.
2. Kinin contents in the samples were almost zero for 5 or 6 days after stopping of the oral hygiene, and then it increased gradually for the following 4 or 5 days. The oral hygiene procedure was resumed at 10 days, and after that kinin contents decreased rapidly until these were not measured in the samples.
3. Bradykininase activities in the samples decreased gradually after the oral hygiene was stopped, and these enzyme activities disappeared at 4-5 days after stopping of the oral hygiene. But, after the resume of oral hygiene, the enzyme activities rapidly increased to the level of the first days of the experiment.
