抄録
The aim of this experiments was to detect the activity of UDP-glucose dehydrogenase and β-glucuronidase and investigate its dynamics in the development of experimental marginal periodontitis in the dogs.
Marginal periodontitis was induced with silk ligatures placed around the 1st molar and 4th premolar at the left jaw bellow the gingival margin. The right jaw was kept clean without ligatures as a control. On day 1 and 2 after ligatures, marked signs of acute gingival inflammation developed. On day 14, marginal periodontitis developed.
UDP-glucose dehydrogenase activity was detected by the method described by Strominger et al. and β-glucuronidase activity was detected by the method described by Fishman.
The UDP-glucose dehydrogenase and β-glucuronidase activities were detected in the gingival tissue of the dogs which developed in gingivitis and periodontitis experimentally with silk ligature and compared with the histopathological findings.
β-glucuronidase activity increased gradually as gingivitis developed and reached the maximum on day 10 after ligature. The activity was approximate 1.6 relative to that in the healthy gingiva.
UDP-glucose dehydrogenase activity decreased on day 1 and 2 after ligature, but reached the maximum on day 10 after ligature. The activity was approximate 2.4 times as much as that in the healthy gingiva. These findings suggested that UDP-glucose dehydrogenase and β-glucuronidase might play an important role in gingival glycosaminoglycan metabolism in the development of gingivitis and periodontitis in dogs.
