Enhanced two-photon excitation fluorescence by ultrafast intensity fluctuations and its application

Yujiro Eto

doi:10.11470/photo.240213

Photonics Review

Online ISSN : 2758-0059

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Enhanced two-photon excitation fluorescence by ultrafast intensity fluctuations and its application

Yujiro Eto

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Keywords: two-photon excitation microscopy, ultrafast intensity fluctuations, parametric down-conversion, photon bunching, green fluorescent proteins, brain tissue

JOURNAL OPEN ACCESS

2024 Volume 2024 Article ID: 240213

DOI https://doi.org/10.11470/photo.240213

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Abstract

We show that the ultrafast intensity fluctuations produced by parametric generator enhance the two-photon fluorescence from green fluorescent protein (GFP) solution by a factor of 1.9. We also show that two-photon fluorescence imaging of a GFP-expressing mouse brain can be readily implemented using parametric generator with such an enhancement effect.

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