Fluorometric Measurement of Adenosine 5'-Triphosphate Using Exonuclease V Activity

Abstract

A new method of a fluorometric measurement of adenosine 5'-triphosphate (ATP) based on the coupling of exonuclease V and a fluorescence-labeled DNA probe was developed. Quantification of micromolar-order ATP and real-time measurement of the activity of the ATP-generating enzyme were performed. This method provides a simple fluorometric assay of ATP and ATP-related enzymes using easily available materials.