Permeation Control Using Enzymatic Reaction of GOD Immobilized onto Oxygen Plasma-pretreated Expanded PTFE Films Grafted with Acrylic Acid

Abstract

The film of expanded polytetrafluoroethylene (ePTFE) grafted with acrylic acid (ePTFE-g-PAA) was prepared by the combined use of oxygen plasma treatment and photografting technique changing plasma-treated time, monomer concentration and UV irradiation time. The permeation of insulin through the ePTFE-g-PAA film can be controlled by the pH change in the medium. Since GOD immobilized onto ePTFE-g-PAA film through covalent bonding can enzymatically react with glucose, we have tried to control the permeation of insulin through the GOD immobilized ePTFE-g-PAA film due to pH lowering in medium.