Constitutive Activity of Inwardly Rectifying K⁺ Channel at Physiological [Ca]_i Is Mediated by Ca²⁺/CaMK II Pathway in Opossum Kidney Proximal Tubule Cells

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Using patch-clamp technique, we studied the role of Ca²⁺/calmodulin kinase II (CaMK II)–mediated phosphorylation process on the K⁺ channel with an inward conductance of 90 pS in opossum kidney proximal tubule cells (OKPC). The intracellular Ca²⁺ concentration ([Ca]_i) was measured by use of the fluorescent dye fura 2. The following results were obtained: (i) In cell-attached patches, the channel activity was inhibited by a decrease in [Ca]_i induced by perfusion with low Ca²⁺ (10⁻⁸ M), La³⁺ (100 µM) or EGTA/AM (100 µM) contained in the bath solution. The application of KN-62 (10 µM) or KN-93 (5 µM), inhibitors of CaMK II, also inhibited the channel activity. (ii) The membrane potential measured with nystatin-perforated patches was significantly decreased by the fall in [Ca]_i induced by the perfusion with EGTA- or La³⁺-containing solution. In addition, the application of KN-62 (10 µM) or KN-93 (5 µM) to the bath significantly decreased the membrane potential. (iii) In inside-out patches, the channel activity was significantly stimulated by the application of CaMK II (300 pm) at 10⁻⁷ M Ca²⁺ in the bath. Furthermore, the application of KN-62 (10 µM) to the bath significantly decreased the channel activity. Our findings show that the constitutive activity of inwardly rectifying K⁺ channel at physiological [Ca]_i is mediated by Ca²⁺/CaMK II pathway in OKPC.