Immunochemical Studies on Phage-mediated Antigens in Salmonella.

I Absorption of Factor Serum by Sugars

抄録

Inhibition experiments by sugars were carried out on hemagglutination of bacterial polysaccharide-coated rabbit red Table II. Inhibition by sugars of hemagglutination of polysaccharide-coated rabbit red cells by factor sera and precipitation of bacterial polysaccharides by factor sera cells by factor serum and on precipitation of bacterial polysaccharide by factor serum, and the following results were obtained concerning the antigenic determinants of phage-mediated antigens in Salmonella:
1. The antigenic determinant of somatic antigen 1, 7)13) which is produced in Salmonella groups A, B, and D by the phage conversion, is considered to be α-glucosyl-(1→6)-galactose-mannose-. The antigenic determinant of somatic antigen 12, 13) which is related to the infection of this phage, was assumed by Stocker, Staub, Tinelli, and Kopacka3) to be α-glucose-(1→4)-galactose-mannose-rhamnose. It is consequently presumed that the difference between somatic antigens 1 and 12 would be only the difference in the form of combination between glucose and galactose.
2. In Salmonella group E, somatic antigen 3, 10 is converted into 3, 15 by means of phage ε 15.8) Both the phage-mediated antigen 15 and the original antigen 10 are considered to have β-galactosyl-(1→4 or 1→6)-glucose-mannose- as their determinants, and it is difficult to distinguish them from each other. Furthermore, in Salmonella group E, antigenic conversion takes place from 3, 15 into (3), (15), 34 by means of phage ε 34.10) The antigenic determinant of the phage-mediated antigen 34 is considered to be α-glucosyl-(1→4)-[galactose or glucose]-. In this way, there is the difference between the somatic antigen 15 and the somatic antigen 34 in the end determinant.
3. In Salmonella group C, somatic antigen 20 is added to 6, 8 by means of phage.9) And this phage-mediated antigen 20 is considered to have N-acetylglucosaminoyl-(1→4 or 1→6)-glucose as its determinant.