Abstract
The chromosomal distribution of the NORs in three species with 2n=48 (Acheilognathus lanceolatus, A. limbatus, and Rhodeus ocellatus ocellatus) and three species with 2n=44 (A. cyanostigma, A. tabira tabira, and A. rhombeus) in Rhodeinae, was investigated using the silver staining technique. In the species with the same chromosome number, the NORs are located in different chromosomes despite of the karyotypic similarity. These results suggest that the alternation of the locations of NORs have played an important role to the species differentiation in this subfamily.