Abstract
Four kinds of cell lines (OST1, OST1-M, OST2, OST3) were established from human osteosarcoma through the transplantation into nude mice of biopsied tumors. OST1 and OST1-M cells were respectively derived from primary and metastatic lesions of a patient. The original cells for OST1 were sampled before the therapy, while those for OST1-M were extracted after the chemotherapy and radiotherapy. Q-banding analysis showed that the chromosomal composition was different between the two cell lines. Also, the marker chromosomes in OST1-M cells contained a number of abnormally stretched one which probably resulted from gene amplification.
