Abstract
Urinary low molecular weight proteins were studied on 4 cases of glomerular proteinuric patients physicochemically and immunologically. Immunoelectrophoretic analysis of those urinary low molecular proteins demonstrated the antigenic properties of albumin, α1-antitrypsin, transfferin, γ-globulin and Bence Jones protein. Urinary low molecular albumin and γ-globulin (IgG) were separated and purified by gel filtration and/or affinity chromatography. The approximate molecular weight of the albumin and γ-globulin were 10,000 and 12,000 respectively by ultracentrifugal analysis. The antigenicity of urinary low molecular albumin was confirmed in experiments of rabbit. Urinary low molecular weight γ-globulin contained IgG, Fc, Fab and light chain of immunoglobulin.
