Juntendo Medical Journal
Online ISSN : 2188-2134
Print ISSN : 0022-6769
ISSN-L : 0022-6769
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Volume 22, Issue 3
Displaying 1-13 of 13 articles from this issue
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Contents
  • A Study on the Urinary Low Molecular Proteins
    MIKIO MORI
    1976 Volume 22 Issue 3 Pages 286-296
    Published: September 10, 1976
    Released on J-STAGE: November 21, 2014
    DOIhttps://doi.org/10.14789/pjmj.22.286
    JOURNAL FREE ACCESS
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    Urinary low molecular weight proteins were studied on 4 cases of glomerular proteinuric patients physicochemically and immunologically. Immunoelectrophoretic analysis of those urinary low molecular proteins demonstrated the antigenic properties of albumin, α1-antitrypsin, transfferin, γ-globulin and Bence Jones protein. Urinary low molecular albumin and γ-globulin (IgG) were separated and purified by gel filtration and/or affinity chromatography. The approximate molecular weight of the albumin and γ-globulin were 10,000 and 12,000 respectively by ultracentrifugal analysis. The antigenicity of urinary low molecular albumin was confirmed in experiments of rabbit. Urinary low molecular weight γ-globulin contained IgG, Fc, Fab and light chain of immunoglobulin.
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  • Local Conformational Changes of the Myosin Molecule Induced by Mg2+ and ATP
    TOSHIKO MIMURA
    1976 Volume 22 Issue 3 Pages 297-309
    Published: September 10, 1976
    Released on J-STAGE: November 21, 2014
    DOIhttps://doi.org/10.14789/pjmj.22.297
    JOURNAL FREE ACCESS
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    In order to detect the local conformational changes in the myosin molecule induced by Mg2+ and nucleotides, we studied the changes through tryptic digestion of myosin in its ATPase activity, in its interaction with actin and in the pattern of its digestion products. The following results were obtained. 1) Mg2+-and Ca2+-ATPase activities of myosin showed a biphasic response-- first activated and then inhlbited--during the course of digestion. 2) Mg2+ slightly accelerated tryptic peptide-bond cleavage occuring in the active site of ATPase. ATP and ITP markedly increased this effect of Mg2+. 3) The activation of the Mg2+-ATPase activitiy by actin was quickly lost by tryptic digestion regardless of the degree of inactivation of myosin ATPase itself. Mg2+-ATP in the digestion medium slightly increased the change mentioned above. 4) The ability of myosin to accelerate G-F transformation of actin was found to be more resistant to tryptic digestion than its catalytic activity. In this respect Mg2+-ATP protected myosin against digestion. 5) Subfragments 1 and 2 were formed by tryptic digestion from myosin in the absence of Mg2+, but hardly obtained in the presence of Mg2+. Nucleotides, KCl concentration and actin added to the system did not affect this cutting-off of the head at all. Judging from these experimental data, sites of tryptic attack in the myosin molecule and the conformations around these sites were discussed.
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  • A Case of Giant Mediastinal Tumor Accompanied with Hypoglycemic Attacks
    KAZUO SHIMIZU, SUSUMU HANZAWA, TAKASHI SASYO, HIDETOSHI KUDO, HIROAKI ...
    1976 Volume 22 Issue 3 Pages 310-316
    Published: September 10, 1976
    Released on J-STAGE: November 21, 2014
    DOIhttps://doi.org/10.14789/pjmj.22.310
    JOURNAL FREE ACCESS
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    Since Goege et al (1930) at first reported a case of giant fibrosarcoma with hypoglycemic attacks, many similar cases have been reported throughout the world. In all cases hypoglycemia had disappeared after the surgical removal of the tumor. In this paper a case of giant mediastinal tumor with hypoglycemic attacks is presented and the pathological mechanism of hypoglycemia due to giant tumor is discussed. The patient was a 64-year-male. On january, 1969, he complained dyspnea and asthma attacks and 2 months later, he was admitted in to our hospital. X-ray findings of the chest showed a giant mediastial tumor and by thoracentesis 300 ml of transudate was obtained. Because the patient evaded the surgical operation, he was treated conservatively and discharged with marked improvement 4 months after admission. Thereafter he was admitted 2 times because of the episodes of dyspnea and cyanosis. In a early morning 12 months after the onset, he had suddenly delirium and irritability and was admitted. After taking breakfast these symptoms completely disappeared and laboratory tests revealed low levels of blood glucose and IRI. The patient finally consented to remove the tumor surgically. The tumor was fortunately resected without residual. The resected tumor was 20 × 21 × 15 cm in size, weighed 3,100 g medullary and encapsulated. The pathological diagnosis of the tumor was fibromyoma. After the operation all clinical symptoms and abnormal laboratory findings disappeared and no recurrence was observed for 28 months. Concerning the cause of hypoglycemic attaks associated with such a giant tumor, although many theories have been postulated, the authors would consider that the hypoglycemia should be attributed to the increasing glucose consumption and releasing a inhibitor of insulinase from the tumor in our case.
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