Abstract
The methods to isolate polymorphonuclear leukocytes (PMNs) in good yield from human venous blood, rabbit arterial blood and rabbit peritoneal exudates were studied, and the properties of PMNs obtained were investigated.
1. The leukocyte preparations from human venous blood contained in PMNs 93-96 per cent, the yield of cells was 40-60 per cent and the viability of cells was 95-98 per cent. The preparationns from rabbit peritoneal exudates contained in PMNs 93-97 per cent and the viability of cells was 84-90 per cent. 2-8 × 108 PMNs were obtained from a rabbit weighing 2.5-3.0 kg.
2. Tris buffer solution used as a cell suspension medium caused leukocytes clumping whereas this clumping was completely avoided by using phosphate buffered saline solution.
3. Human PMNs were more stable than rabbit PMNs for mechanical stimuli, judging from the trypan blue exclusion test and the release of lactate dehydrogenase into extracellular medium.
4.β-Glucuronidase, peroxidase, lysozyme and lactate dehydrogenase contents were nearly equal between hnman and rabbit PMNs. On the other hand, acid phosphatase activity in human PMNs was about 12 times higher than that in rabbit PMNs, whereas in the activity of alkaline phosphatase rabbit PMNs showed 6-fold higher than human PMNs. However, the total activity of phosphases, namely the sum of acid and alkaline phosphatases, was the same.
5. No differences in the membrane stability and enzyme contents were observed between PMNs obtained from rabbit arterial blood and peritoneal exudates.
