Abstract
(1) A study was undertaken for the purpose of determining if Candida albicans would develop resistance in vitro to polymyxin B, phenyl mercury acetate (mercetat), trichomycin, and brilliant green.It was found that Candida albicans does not develop any resistance to the three drugs except polymyxin B. With polymyxin B only, there was a moderate build up of resistance (8 folds).
(2) The resistant strain to polymyxin B showed cross resistance with colistin and was more sensitive to trichomycin than parent strain.
(3) Morphologically, the resistant strain seemed to be little alterable.It was found that resistant strain has no ability to produce chlamydospore and shows lowe rability in fermentation comparing with parent strain.
(4) The growth of resiStant strain seemed to be the same or slightly better than parent strain.
(5) The pathogenicity of resistant strain to mice was considered to be slightly stronger than that of parent strain.
(6) On the pathologic and histologic examination of mice organs, it was found that resistant strain brings about stronger necrosis of tissue and cellular infiltration particulary in heart than parent strain.
On the examination of morphology of fungi in tissue, mainly pseudomycelial form was found in mice inoculated with retistant strain, in con'rast to yeast form seen in those of parent strain.
(7) The resistant strain recovered its sensitivity similar to that of the parent strain after 14 trrnsfers with one month interval in drug·free medium.
