Abstract
Objective : The dog is known to be sensitive to various allergens and many studies about the relationship between canine IgE and allergic diseases have been reported. However, the analysis of canine IgE receptor has been hardly reported. Here we produced and characterized two monoclonal antibodies (mAbs) that recognized canine high affinity IgE receptor (FcεRI).
Materials, Methods and Results : By flow cytometric analysis, these mAbs bound to transfectants of canine FcεRI but not to CHO cells as used as parent cells. Immunoprecipitation and immunoblot studies with these mAbs showed characteristic bands that were consistent with the molecular weight of rat or human FcεRI previously reported. These mAbs inhibited binding of IgE to canine FcεRI transfectants. Functionally these mAbs caused histamine release from canine mastocytoma cells. Transfection assay showed that canine FcεRI could be expressed on cell surfaces in combination with only α and γ chains, which is the same combination shown in studies of human FcεRI. Conclusions : Our new antibodies are expected to be useful for further analysis of canine FcεRI.
