Juntendo Medical Journal
Online ISSN : 2188-2134
Print ISSN : 0022-6769
ISSN-L : 0022-6769
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Volume 57, Issue 2
Displaying 1-16 of 16 articles from this issue
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Contents
  • Establishment of monoclonal antibodies that recognize canine high affinity IgE receptor (FcεRI) and activate canine mastocytoma cells
    OSAMU MURAKAMI, RYO GOITSUKA, KOHJI MARUO, CHISEI RA, KO OKUMURA
    2011 Volume 57 Issue 2 Pages 125-132
    Published: April 30, 2011
    Released on J-STAGE: November 21, 2014
    DOIhttps://doi.org/10.14789/pjmj.57.125
    JOURNAL FREE ACCESS
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    Objective : The dog is known to be sensitive to various allergens and many studies about the relationship between canine IgE and allergic diseases have been reported. However, the analysis of canine IgE receptor has been hardly reported. Here we produced and characterized two monoclonal antibodies (mAbs) that recognized canine high affinity IgE receptor (FcεRI). Materials, Methods and Results : By flow cytometric analysis, these mAbs bound to transfectants of canine FcεRI but not to CHO cells as used as parent cells. Immunoprecipitation and immunoblot studies with these mAbs showed characteristic bands that were consistent with the molecular weight of rat or human FcεRI previously reported. These mAbs inhibited binding of IgE to canine FcεRI transfectants. Functionally these mAbs caused histamine release from canine mastocytoma cells. Transfection assay showed that canine FcεRI could be expressed on cell surfaces in combination with only α and γ chains, which is the same combination shown in studies of human FcεRI. Conclusions : Our new antibodies are expected to be useful for further analysis of canine FcεRI.
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  • Preventive Effects of Erythropoietin on Peritoneal Hypoxia and Fibrosis in Rats with Chlorhexidine Gluconate-induced Peritoneal Fibrosis
    JIRO INUMA, CHIEKO HAMADA, TETSUTARO SHIMAOKA, YOSHIMI SEKIGUCHI, YOKO ...
    2011 Volume 57 Issue 2 Pages 133-143
    Published: April 30, 2011
    Released on J-STAGE: November 21, 2014
    DOIhttps://doi.org/10.14789/pjmj.57.133
    JOURNAL FREE ACCESS
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    Background: Vasculopathy is one of the characteristic findings in long-term peritoneal dialysis (PD) patients. Erythropoietin (Epo) used to treat renal anemia may promote angiogenesis. The present study evaluated anti-fibrotic effects of Epo in the rat with chlorhexidine gluconate (CG) -induced peritoneal sclerosis. Methods: Twenty-four Sprague-Dawley rats at 8 weeks of age were divided into four groups. The rats were intraperitoneally administered CG and Epo (CG+Epo group), CG alone (CG group) or Epo alone (Epo group) for 28 days. A control group was administered ethanol and vehicle. Expressions of hypoxia-inducible factor-1a (HIF-1a), erythropoietin receptor (EpoR) and pimonidazole were evaluated by immunohistochemistry. HIF-1a, vascular endothelial growth factor (VEGF), angiopoietin-1 (Ang-1), angiopoietin-2 (Ang-2) and connective tissue growth factor (CTGF) were examined by real-time PCR. Double staining was performed for CD34 and a-smooth muscle actin, and the microvessel density (MVD) and microvessel pericyte coverage index (MPI) were calculated. Results: Peritoneal fibrosis stimulated by CG was suppressed by Epo administration, and hematocrit in the CG+Epo group was significantly higher than that in the CG group. The numbers of HIF-1a, EpoR and pimonidazole expressing cells in the CG+Epo group were lower than those in the CG group. The expressions of HIF-1a, VEGF, Ang-2 and CTGF mRNA were increased in the CG group although they were reduced in the CG+Epo group. The Ang-1 mRNA expression in the CG group was lower than that in the CG+Epo group. Since MVD and MPI were significantly higher in the CG+Epo group than in the CG group, a sufficient vascular network may be established in the CG+Epo group. Conclusions: Epo may play an important role in preventing peritoneal fibrosis via improvement of hypoxia by elevation of hematocrit, establishment of a sufficient microcirculatory network and decrease of CTGF production by inhibition of HIF-1a expression in this rat model.
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  • Usefulness of separator seals in double-bag amino acid containers
    HIDEKO AIDA, YASUO SUMOMOZAWA, TAKEHIKO YUKISHITA, KEIKO LEE, MAKI USU ...
    2011 Volume 57 Issue 2 Pages 144-150
    Published: April 30, 2011
    Released on J-STAGE: November 21, 2014
    DOIhttps://doi.org/10.14789/pjmj.57.144
    JOURNAL FREE ACCESS
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    Objective : Double-bag amino acid container is a convenient and innovative product designed to prevent the Maillard reaction by partitioning amino acids and glucose into two compartments until administration. However, it does not eliminate the human error of forgetting to break the partitions before administration. In this study, we compared the external separator seal with an internal one to determine the usefulness of these built-in devises, and to also determine their effectiveness in preventing erroneous administration due to a lack of attention. Materials and Methods : Ten registered nurses with no prior experience in administering a single drug using a double-bag system operated the separator seals of three different products : Bfluid (Otsuka Pharmaceutical Co., Ltd.), Amigrand (Terumo Corporation and Mitsubishi Tanabe Pharma Corporation), and Paresafe (Ajinomoto Co., Inc.). Each participant was assigned an observer that measured the time and evaluated the participant's handling of the separator seals.The labels on the double-bag that the participants used to visually assess the status of the partitions were also assessed.In addition, 132 registered nurses with proficiency in handling double-bag preparations were surveyed for 6 months. Results : We found that the internal separator seal (Amigrand) prevented human error more frequently than external seals (Bfluid, Paresafe). Seven participants on Bfluid and one on Paresafe were able to remove the separator seal by hand, irrespective of breaking the partitions.The labels on the double-bag consisted of 41 items on Bfluid (68.3%), 36 items on Amigrand (51.4%), and 26 items on Paresafe (37.2%). A significant difference was found between “ease of breaking the partitions” (p=0.0047) and “mixed response regarding the handling of separator seals” (p=0.0165) -with respect to the visibility of labels.Our survey showed that the separator seal reduced the incidence of administering unopened preparations in 68.2% of participants receiving Amigrand, 58.1% receiving Paresafe, and 51.0% receiving Bfluid.Although a significant difference was not detected among the three products, Amigrand received the highest rating. Conclusions : We conclude that Amigrand is the most secure double-bag preparation among the three products because its separator seal cannot be deliberately removed and the labels on the bag permit smooth breaking of the partitions.
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  • Preliminary Experience with Immediate Postoperative Prosthesis Using Silicone Socket (IPOPSS) for Lower Limb Amputation
    ATSUHIKO TERAKADO, MASANORI NAGAOKA, HISASHI KUROSAWA, TOMOAKI TORIGOE ...
    2011 Volume 57 Issue 2 Pages 151-157
    Published: April 30, 2011
    Released on J-STAGE: November 21, 2014
    DOIhttps://doi.org/10.14789/pjmj.57.151
    JOURNAL FREE ACCESS
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    Objective: Soft dressing using elastic bandages has been generally applied after lower limb amputation. However, due to stump edema, a long period has been required until measurement could be obtained for the production of a temporary prosthesis. As a result, hospital stay was prolonged. We have developed a new system using Immediate Post-Operative Prosthesis using a Silicone Socket (IPOPSS). This IPOPSS system allows both easy wound-dressing change and frequent observation by removing the socket, and early rehabilitation by immediate postoperative prosthetic fitting. To investigate the hospitalization period, we compared soft dressing with the IPOPSS method. Materials and Methods: The subjects were 21 patients (22 affected limbs) who were postoperatively managed with IPOPSS between January 2003 and January 2005. The cause of amputation was peripheral vascular disease in 7 limbs, diabetic ulcer in 6, malignant tumor in 5, and infection in 4. Between 1978 and 2002, we managed 45 limbs by applying soft dressing postoperatively. Results: Concerning the hospitalization period, IPOPSS fitting was performed after lower limb amputation, the mean interval between surgery and measurement was 35 days, and that between surgery and discharge was 49 days. Using soft dressing, the mean interval between surgery and measurement was 53 days, and that between surgery and discharge was 116 days. Conclusions: The hospitalization period was shortened by IPOPSS compared with the conventional postoperative management method.
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