Supplementary Studies on the Serological Classification of Yeast

II. Antigenic Analyses of Four Species of the Genera Hansenula and Pichia

Abstract

Amemiya, Nishikawa and Fukazawa reported respectively that there are serological cross reactions among many species of the genera Candida, Hansenula and pichia. Four species of the genera Hansenula and Pichia, of which strains had not been established the antigenic strutures, were obtained for antigen analysis. The experiments were carried out througth the slide agglutination method using monospecific or absorbed antisera homologous to the antigens of the genus Candida. Then, an antiserum of a species was absorbed repeatedly with heated cells of several species in order to find a new antigen, until the absorbed antiserum was exhausted. Antigenic structures of the species were confirmed by the reciprocal absorptions. Hewever no new thermostable and thermoladile antigen was found. Antigenic structures of the species as follows : H. suaveolens has the thermostable antigens, 1, 2, 14, 15, 16, 17 and 20. H. holstii has antigens, 1, 2, 3, 13, 14, and 15. H. matritensis has the antigens 1, 2, 3, 4, 9 and 14, and the antigenic structure is the same to that of Torulopsis globosa. P. fermentans has the aatigens, 1, 2, 5 and 11. All he species have not any thermolabile antigen.