Abstract
Intracellular structures of Saccharomyces cerevisiae (diploid and haploid strains) and Candida robusta (proved to be triploid strain of Saccharomyces by Takahashi, 1959) were investigated with several nuclear and mitochondrial staining dye solutions.
Staining procedure used were, HCl-fuchsin, HCl-thionin, HCl-toluidine blue, ribonuclease digestion method, supravital staining with methyl green, neutral red, janus green and toluidne blue, and many combinations with these procedures.
Results obtained were summarized as follows : 1) So called cell nucleus of the yeasts were obviuosly different from mitochondria. 2) Number of the structure stained by nuclear staining dyes did not increase parallely to the ploidy. 3) Nuclear substance exist in the area surroundig the vacuole, or diffusely dispersed nuclear substance accumulate in the perivacuolar area in the process of the staining procedure. And consequently shrinkage of the vacuole occured resusting in formation of chromosome-like nuclear configurations.
