Abstract
I have studied the acute, subacute and the chronic toxic effects of NMO (Nitromin: Methyl-bis (β-chloroethylamine) N-oxide hydrochloride), especially in the view point of haematology and patho-ana-tohistology, with healthy rabbits, rats and mice. Then, experimental studies have been undertaken in the hope of establishing the develop of a resistant subline of Yoshida-sarcoma by connective passage of sarcoma in Oosawa strain rats receiving continous injecteon of NMO.: following result were performed.
1) During the succesive injection of NMO, leucopenia and following anemia were noticed, at the same time anatomical findings also showed severe panmyelophthisis, desolution of spermatogenesis, atrophy of spleen with degeneration, lymphrtic gland and thymus and ulcer with edema in the large intestine.
But some of them showed remarkable recovery in blood picture, and their bone marrow, spleen and lymphatic gland were rather more hyplastic and leucemoid.
2) According to marked shortning of the survival time of rats inoculation with sarcoma inspite of the injection of NMO, it has been demonstrated that a resistant subline of sarcoma to NMO has been developed relativ easely, and that the subline has showed a tendency towards more rapid growth receiving injection of NMO.
3) Some relationship were recognized between the development of resistance to NMO in bone mallow, testis and intestinalepithelium of healthy animals, and the develpment of resistant variant of Yoshida-sarcoma. These resistance were thought as a variation of reaction-mood of the cells to the NMO during the continous injection of the agent. These findings favor the assumption that inhibitiv effect in cancerous growth can be changed to its growth promoting effect in NMO-treatment.
