2010 年 27 巻 2 号 p. 211-215
An efficient method for in vitro propagation has been developed for pecan [Carya illinoinensis (Wangenh) K. Koch], a highly recalcitrant but commercially important fruit species. Nodal explants from in vitro grown cultivars, ‘Desirable’ and ‘Cape Fear’, were cultured on modified liquid woody plant medium (WPM) supplemented with 2% glucose and different concentrations of 6-benzylaminopurine (BAP; 0.44–44.39 μM). At least nine multiple shoots per explants were induced on modified WPM containing 13.32 μM BAP after three weeks of culture. The efficiency of shoot induction was over 95% in both ‘Cape Fear’ and ‘Desirable’ cultivars. The multiple shoots were proliferated and/or elongated on plant growth regulator free liquid WPM. Subsequently, the multiple shoots were separated and successfully rooted in liquid WPM containing 49.20 μM indole-3-butyric acid. The efficiency of rooting was over 90% in both ‘Cape Fear’ and ‘Desirable’ cultivar. The pecan plantlets were initially transferred to peat pellets and subsequently to the greenhouse. This is a simple and efficient protocol that may be used to propagate pecan plants through tissue culture.