Shoot Regeneration from Explants of Seedstalk Developed in vitro in Chinese Cabbage

抄録

A convenient method for producing shoot regeneration has been developed for the Chinese cabbage (Brassica campestris L.). Internodes of seedstalks which were developed from the sterilized seeds by low temperature treatment in vitro were used to induce shoot regeneration. The shoot was stably induced when the seedstalk internode sections were incubated in MS medium containing 1mgl^-1 naphthaleneacetic acid (NAA) and 5mgl^-1 benzyladenine (BA), though the frequency was about 16%. Similar results were also obtained from the explants grown in a greenhouse as well as in a plant box. These results suggest that this culture system may be available for micropropagetion, genetic transformation and mutation breeding.