抄録
Genetically transformed plants of virus-resistant genotype of Gossypium hirsutum L. CIM-443 were generated by using Agrobacterium and particle bombardment method. An insecticidal cry1Ab gene derived from Bacillus thuringiensis was introduced in the plants. Inoculated tissues were selected on MS medium containing kanamycin. The resultant plants showed protection against Lepidopteran insect Helicoverpa armigera. Molecular analysis showed the presence of cry1Ab gene in the genome of transformed plant. Immunological analysis of the transgenic plants indicated expression of Cry1Ab protein upto 100-120ng per mg of fresh leaf weight.
