2000 年 17 巻 2 号 p. 119-125
The hydraulic properties of tissue-cultured soybean (Glycine max [L.] Merr.) embryos grown on trehalose-containing culture media were investigated. Water potentials of culture media ranged from -0.13 to -0.65MPa when sucrose and/or trehalose concentrations were altered. The wall extensibility was greatly larger than hydraulic conductance, and the size of the effective turgor was much smaller than that of the growth-induced water potential, indicating that hydraulic conductance and the growth-induced water potential were predominantly regulating growth of soybean embryos under tissue culture conditions. The hydraulic conductance of soybean embryos grown on trehalose-containing media became smaller than that in sucrose-containing media. When both sucrose and trehalose were added to the culture media, the hydraulic conductance became an intermediate between only sucrose-containing and only trehalose-containing treatments. It is suggested that trehalose might reduce hydraulic conductance in soybean embryos, resulting in growth retardation of soybean embryos.