Artificially-synthesized Intergeneric Chimera between Brassica oleracea and Raphanus sativus by In Vitro Grafting

抄録

The first intergeneric chimeras between radish and cabbage were successfully synthesized by in vitro grafting method. Regenerated seventy seven chimeras from the original sectorial chimera were investigated on morphological, physiological natures comparing with other interspecific chimeras of Brassica. Chimeral structures were justified and totally classified based on the morphological characteristics, isozymatic band patterns and PCR analysis. In those chimeras, putative four-layered chimeral plants were also found for two vegetatively-propagated generations with general three-layered chimeras. Physiological interactions were typically recognized such as in flowering date, pollen fertility, and pod and seed set. However, genetic changes were not found in the progeny derived from the crosses with both parents.