Morphological changes in Golgi apparatus induced by brefeldin A treatment in tobacco protoplasts

Takako S. Kaneko; Rei Watanabe; Mamiko Sato; Masako Osumi; Akira Takatsuki

doi:10.5685/plmorphol.6.1

Takako S. Kaneko, Rei Watanabe, Mamiko Sato, Masako Osumi, Akira Takatsuki

著者情報

キーワード: Nicotiana tabacum L. var. Xanthi, tobacco cells, tobacco protoplasts, Golgi apparatus, brefeldin A

ジャーナルフリー

1994 年 6 巻 1 号 p. 1-11

DOI https://doi.org/10.5685/plmorphol.6.1

詳細

Published: 1994 Received: 1994/09/13 Available on J-STAGE: 2010/06/28 Accepted: - Advance online publication: - Revised: -
訂正情報
訂正日: 2010/06/28 訂正理由: - 訂正箇所: PDFファイル訂正内容: -

訂正日: 2010/06/28 訂正理由: - 訂正箇所: キーワード情報訂正内容: Wrong : Key Words: Nicotiana tabacum L. var. Xanthi; tobacco cells; tobacco protoplasts; Golgi apparatus; brefeldin A.
Right : Nicotiana tabacum L. var. Xanthi, tobacco cells, tobacco protoplasts, Golgi apparatus, brefeldin A

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抄録

We investigated the effect of brefeldin A(BFA)on the Golgi apparatus in cultured tobacco protoplasts. Within 3 hr of treatment with brefeldin A(10μg/ml), the diameter of the Golgi cisternae became about four times greater than the control. The expanded cisternae were loosely organized, their tips were sprinkled with ribosomes and the distance between the cisternae increased. No other significant morphological changes were detected, except the appearance of many vacuoles. From these observations it was concluded that BFA causes morphological changes in Golgi apparatus.

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