抄録
The sequential mold-cast technique was employed to examine the development of abaxial epidermis, including stomatal complex and multicellular trichomes, of Vigna radiata(Leguminosae). Sequential impressions were made of the abaxial surface of the first foliage leaf at daily intervals to follow leaf development. Casts were made by filling molds with epoxy adhesive, which was polymerized at 60°C for 1hr. Hardened casts were sputtercoated with gold, and viewed with SEM. When very young, the abaxial leaf-epidermis is thoroughly covered with dense wax deposits, which make trichomes and developing stomata invisible from the surface. As the leaf grow, the wax deposits on the epidermis appears to be reduced in amount. Trichome production has finished by the time when the leaf begins to unfold, while stomata are continuously produced until relatively late stage of leaf development.
