The scientific technique such as genetic engineering is important in both classification of the cyanobacteri a and analysis of the evolution process. The purpose of this research was to decide the base sequence of such cyanobacteria by 16S ribosomal RNA for the classification and its applications.
From the result of the experiments, the base sequences of musty odor and toxin producing cyanobacteria were determined, and cyanobacteria have many species specificity. Moreover, cyanobacteria were identified by PCR and FISH methods using proves with different species specificity. Musty odor producing cyanobacteria and toxic compound producing cyanobacteria were divided into two groups in phylogenetic tree. The former also could divide two groups, one was 2-methylisoborneol producing group and another was geosmin producing group. It is interesting when evolution is argued.
It was found out that the various forms of Phormidium tenue existed in Kamafusa Lake from the base sequences and its phylogenetic tree. For example, dark-brown P. tenue does not change from the indigo-blue P. tenue. Furthermore, occurrence of musty odor of summer and winter is caused by different P. tenue.