The natural environmental investigations were carried out on the source of Shinano river and its basin. At the beginning, the method of natural environmental investigation was simply represented.In this report, in particular, the authors clarifly the ture character of source of Shinano river.Moreover, the data of chemical analysis about water qualities of source zone of Shinano river and natural water in its basin and Kanto area are shown, and the real condition of water environment in the water system of Shinano river is discussed.