抄録
The present study attempted to detect ovulation to facilitate ovulation detection for artificial insemination in a chimpanzee using hemagglutination inhibition test for urinary luteinizing hormone/chronic gonadtropin (LH/CG test) and hemagglutination test for urinary estrogen (E test). We detected an estrogen peak during the follicular phase and an LH surge by this method. These tests allowed for an estimation of ovulation time and were more reliable than the method of evaluating by the sex skin swelling pattern. After the artificial insemination, we carried out pregnancy diagnosis by LH/CG test and E test. LH/CG and E levels increased 13 days and 7 days after the LH surge, respectively. We confirmed that the combination of both tests could be useful for indirect diagnosis of pregnancy as early as the second week after the LH surge. We used ultrasonography for definitive diagnosis of pregnancy. The fetal biparietal diameter was 27mm at 86 days gestation (from the LH surge). We measured the heart rate of mother and fetus from 99 days gestation to delivery without anesthesia. The present study suggests that ultrasonography is a safe and noninvasive technique for real-time monitoring of pregnancy.
