抄録
Zinsser and his co-workers (1) devised an agar tissue medium which was reported to be satisfactory for the growth of rickettsiae. This medium was found to be quite satisfactory for the cultivation of both rickettsiae and viruses. Spotted fever rickettsiae (Fitz Patrick, 2), equine encephalomyelitis virus (Fitz Patrick, 3), vaccinia virus (Kurotchkin, 4), herpes virus (Cheever, 5), virus of St. Louis encephalitis (Pang and Zia, 6), virus of psittacosis (Yanamura and Meyer, 7), and Bartonella tyzzeri (Weinman and Pinkerton, 8) were cultivated successfully on this medium. Poleff (9) reported to have cultivated the virus of trachoma on this medium. An attempt was made to cultivate Ricketlsia orientalis by Kanazawa (10) . Takemori (11) cultivated successfully the virus of lymphogranuloma inguinale and rickettsiae of tsutsugamushi disease on agar tissue medium.
Thompson and Coates (12) reported the multiplication of vaccinia virus in deep columns of Maitland medium. Pseudorabies virus (13) and the virus of common cold (14) were also cultivated in deep culture. Hitz (15) cultivated the typhus rickettsiae in test tubes, but the details of his study are not known to us. Yaoi and Arakawa (16) succeeded in cultivating the viruses of vaccinia and lymphogranuloma inguinale in deep cultures by the aeration of the medium. This technique was used successfully by Findlay and MacCallum (17) for the cultivation of yellow fever virus. Takemori (18) studied the propagation of the virus of lymphogranuloma inguinale and typhus rickettsiae in deep columns of Maitland medium without aeration of the medium. Yanamura and Meyer (7) reported also the cultivation of the psittacosis virus in deep culture.
Chumakoff (19) reported the cultivation of the virus of tick-borne encephalitis but we are not aware of the details of his studies.
Since August, 1944, we have attempted to cultivate the virus of tick-borne encephalitis in deep columns of Maitland medium and on agar tissue medium. In August, 1945, our cultures had to be interrupted involuntarily, and therefore, although our studies are not completed, the portion of our studies dealing with the propagation of the virus will be described in this paper.
