抄録
The sex was identified by the polymerase chain reaction (PCR) using DNA extracted from noninvasive samples, such as urine, feces, food wadges, and hair samples, of Pan troglodytes, Pan paniscus, and Macaca fuscata. The Amelogenin gene was successfully amplified by PCR, and X- and Y-specific DNA regions had different lengths. The ZFX and ZFY genes were also amplified by PCR, and restriction fragment length polymorphism (RFLP) depicted different patterns between the sexes. Sex identification using DNA from noninvasive samples obtained from unidentified wild animals will help to study the range and social structure of these species.
